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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for in vitro isolation, induction, expansion, and determination of human natural regulatory T cells and induced regulatory T cells
doi: 10.1016/j.xpro.2022.101740
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Flow Cytometry, Staining, Cell Isolation, Enzyme-linked Immunosorbent Assay, Software, Transferring, Cell Culture, Cell Counting
Journal: Scientific reports
Article Title: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research.
doi: 10.1038/s41598-017-02599-y
Figure Lengend Snippet: Figure 1. Comparative analysis of centrifugal filters for protein and particle recovery of EVs in PBS. (a) 2 mL PBS spiked with GFP-positive EVs was centrifuged at 3,000 g. Concentrate and filtrate were collected. Lysates of filter membranes were used for analysing retained proteins. (b) Particle recovery was analyzed using Nanoparticle tracking analysis (n = 3, *p < 0.0001). (c) Protein recovery was assessed based on protein concentration measurements (n = 3, *p < 0.0001). (d) Retained proteins on the filter membrane were quantified (n = 3, *p < 0.0009). (e) Lysates were used for Western blot immunostaining for GFP. Mean values + SD are indicated. Significant differences were calculated using Student’s t-test with RC 10k as reference. Original immunostaining results are shown in Supplementary Fig. 10. Abbreviations: PBS: phosphate buffered saline. GFP: green fluorescent protein. EV: extracellular vesicle. RC: regenerated cellulose. HY: Hydrosart. PES: polyethersulfone. CTA: cellulose triacetate. WB: Western blot. NTA: Nanoparticle tracking analysis. PCM: protein concentration measurement. RT-qPCR: real time quantitative polymerase chain reaction. EM: electron microscopy.
Article Snippet: Five different centrifugal filters were used: Amicon Ultra-2 10k (UFC201024, Merck Millipore, Billerica, Massachusetts, USA), Amicon Ultra-2 100k (UFC210024, Merck Millipore, Billerica, Massachusetts, USA), Vivaspin 2 PES 10k (VS0201, Sartorius Stedim Biotech GmbH, Goettingen, Germany), Vivaspin 2 CTA 10k (VS02V1, Sartorius Stedim Biotech GmbH, Goettingen, Germany) and
Techniques: Protein Concentration, Membrane, Western Blot, Immunostaining, Saline, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Electron Microscopy
Journal: Scientific reports
Article Title: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research.
doi: 10.1038/s41598-017-02599-y
Figure Lengend Snippet: Figure 3. Comparative analysis of centrifugal filters for protein and particle recovery from plasma and urine. (a) Blood plasma was loaded on SEC column and EV-rich fractions were concentrated using five different centrifugal filters. Urine was concentrated using the same type of filters. (b) Nanoparticle Tracking Analysis (n = 2, *p < 0.05) and (c) Western blot analysis for syntenin-1 was performed on concentrated EV-rich SEC- fractions from plasma (n = 2, *p < 0.04). Western blot analysis was quantified using RC 10k as reference. (d) Concentrated urine was analyzed by Nanoparticle tracking analysis (n = 2, *p < 0.05) and (e) Western blot analysis for syntenin-1 (n = 2, *p < 0.02). Western blot analysis was quantified using RC 10k as reference in (c,e). Mean values + SD are indicated. Significant differences were calculated using Student’s t-test with RC 10k as reference. Original immunostaining results are shown in Supplementary Fig. 10. Abbreviations: SEC: size-exclusion chromatography. EV: extracellular vesicle. RC: regenerated cellulose. HY: Hydrosart. PES: polyethersulfone. CTA: cellulose triacetate. WB: Western blot. NTA: Nanoparticle tracking analysis. PCM: protein concentration measurement.
Article Snippet: Five different centrifugal filters were used: Amicon Ultra-2 10k (UFC201024, Merck Millipore, Billerica, Massachusetts, USA), Amicon Ultra-2 100k (UFC210024, Merck Millipore, Billerica, Massachusetts, USA), Vivaspin 2 PES 10k (VS0201, Sartorius Stedim Biotech GmbH, Goettingen, Germany), Vivaspin 2 CTA 10k (VS02V1, Sartorius Stedim Biotech GmbH, Goettingen, Germany) and
Techniques: Clinical Proteomics, Western Blot, Immunostaining, Size-exclusion Chromatography, Protein Concentration